BIO-DATA

Dr. Kharat Arun Sidram

Professor & Head

M.Sc., SET, Ph.D.PDF-France, USA.

Ramai, 1690 Zadbuked Maidan, Behind BTM., Agalgoan Road, Barshi Dist-Sholapur, Maharashtra, 413 401.

arunkharat2007@rediffmail.com

992-355-5705

9 Years (PG)
7 Years (UG)

Total –22 publications. Of twenty-two, 10 are published research articles, Two research articles are being submitted. Remaining ten are nucleotide submissions at www.ncbi.nlm.nih.gov/nucleotides for details please see Annexure I

M. Phil. : Nil Ph. D.Two being registered

1) Research Project Undertaken: Worked on nine different project as a collaborator or associate investigator. One project is being submitted as Principle Investigator, to Department of Science and Technology, India.

04 National Conferences and 01 International conference

A. Visited Cold Spring Harbor Laboratory, New York, USA, travel funded by the noble prize laureate Dr. J. D. Watson –1998
B. Visited University of Umea, Umea, Sweden, -1999
C. One year teaching cum research work was carried out at University Joseph Fourier, Grenoble France, Funded by Ministry of Science, France. –2000.
D. Visited University of Gdansk, to deliver invited speech, - 2000.
E. Visited University of Toulouse, France, to run a collaborative research, Funded by the same University, -2002
F. Six years post-doctoral research at Rockefeller University, New York, USA, Funded by Pfizer Pharmaceuticals, USA. - 2001-1007.

1) State Bank of India Scholarship for High-school, Higher Secondary School, graduation and post-graduation studies.
2) Department of Biotechnology, India scholarship as research scholar (Ph.D.) at Indian Institute of Science, Bangalore.
3) Obtained Scholarship by Dr. James for travel while attending meeting on Genetics of Bacteria and Phages, 1998 that was held at Cold Spring Harbor, NY, USA.
4) French ministry of science, awarded one year fellowship to do postdoctoral research at Universite Joseph Fourier, Grenoble, France.
5) Participant of “Pneumococcal Resistance Epidemicity and Virulence an International Study (PREVIS)” from January 2004 to December 2006.
6) Reviewed research articles for various journals published in USA and Europe.

Nil

Papers in International meetings

1. Presented a Poster-“Molecular and functional analysis of the β-glucoside utilization genes in Shigella sonnei.” In the discussion meeting on the “Molecular Genetics of bacteria and phages”- 25th August to 29th August 1998, held @Cold Spring Harbor Laboratory, USA. Sponsored by Dr. James Watson, Director, CSHL, New York, USA.
2. Delivered a lecture- “Molecular Evolution of the β-glucoside utilization genes n the Shigella group of organisms.” The 5th Meeting of the students in Evolutionary Biology. 23rd March-26th March 1999. Umea University, Umea, Sweden. Sponsored by department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore.
3. Delivered a lecture- Functional coupling of srtA and gyrA in Streptococcus pneumoniae. Europneumo congress held at Institute of Genetics and recombinant DNA technology, Lisbon, Portugal. Sponsored by Pfizer pharmaceuticals, USA.
4. Delivered a lecture- In vivo differential display of cytokines in CD1 mouse infected with choline independent mutants, held at Institute of Genetics and Recombinant DNA technology, Lisbon, Portugal, Sponsored by Pfizer Pharmaceuticals, USA.
5. Participant of “Pneumococcal Resistance Epidemicity and Virulence an International Study (PREVIS)” from January 2004 to December 2006.

Papers in national meetings

1. Presented a poster- “Molecular and functional characterization of the β-glucoside utilization in the Shigella group of organisms.” In the 67th Annual meeting of the Society of the Society of Biochemists in India,”- 9th December – 12th December 1998. Jawaharlal Nehru University, New Delhi, India.
2. Delivered two invited lectures: National level theoretical workshop on Biotechnology, Mahatma Gandhi’s Mission, Aurangabad, January 3rd and 4th 2008.
3. Presented paper and Invited Lecture: National conference on Recent trends in Biotechnology with reference to Animal Biodiversity, January 28th –30th 2008, at Arts, Commerce Science College, Indapur, Dist- Pune.
4. Presented paper and Invited lecture: National conference on Horizons in Biotechnology, February 8-9th 2008 at Swami Vivekananad College, Udgir.

Guest Lectures

1. Delivered series of guest lectures: Recombinant DNA technology and Genetic transfer in bacteria during 1994 &1995 for the Department of Zoology, Mahatma Phule College, Pimpri, Pune-17.
2. Delivered guest lecture: “Molecular evolution of the β-glucoside utilization genes within a few members of the family Enterobacteriaceae.” 6th August 2000, University of Gdansk, Gdansk, Poland.
3. Delivered guest lecture: The β-glucoside utilization genes, role model to study bacterial evolution and translation fidelity by transposition of IS elements in enteric bacteria. 17th September 2001, Tufts University, Boston, USA.
4. Delivered guest Lecture: Recombinant Bio-technology subject of 21st century. 24th November 2004, Shriman Bhausaheb Zadbuke Mahavidyalaya, Barshi, Sholapur, MS, India.
5. Delivered guest Lecture: Pneumococcal surface and virulence determinants. February 6th 2007. Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, KS, India.
6. Delivered guest Lecture: Recombinant DNA technology –an overview. 26th February 2007. Rajashtri Shahu College, Lature, MS. India.
7. Delivered guest Lecture: Pneumococcal surface and its contribution to virulence. Indian Institute of Science, Bangalore, arranged by Genesis society. February 6th 2007.
8. Delivered Plenary talk: Recent trends in Biotechnology in context of Animal development and Environmental Biology. Arranged by ASC college, Indapur, Pune University, sponsored by Zoologist Society, India, UGC and Pune University.
9. Delivered two guest Lecturers: Role of Sortase and Role of Choline in pneumococcal pathogenesis, Institute of Biosciences, and Technology at MGM, Aurangabad., Jan 31st and February 1st 2008.

Research Publications

1. Arun S. Kharat & S. Mahadevan (1999) “Plasmid mediated Suppression of the mutational activation of the bgl operon of Shigella sonnei” Acta. Biochemica. Polonica 46(4): pp156-167.
. 2. Arun S. Kharat & S. Mahadevan (2000) “Analysis of the β-glucoside utilisation (bgl) genes of and Shigella sonnei: Evolutionary implications for their maintenance in a cryptic state.” Microbiology 146(8) 2039-2049.
3. Arun Sidram Kharat* (2001) “Phenotypic variability of β-glucoside utilization and its correlation to pathogenesis process in a few enteric bacteria.” FEMS Microbiology Letters 199 (2) 241-246.
4. Arun S. Kharat and Alexander Tomasz (2003). “Inactivation of the srtA gene affects localization of surface proteins and decreases adhesion of Streptococcus pneumoniae to human pharyngeal cells in vitro.” Infection and Immunity 71(5) 2758-2565.
5. Arun S. Kharat and Alexander Tomasz (2006). “Drastic reduction in the virulence of Streptococcus pneumoniae expressing type-2 capsular polysaccharide but lacking choline residues in the cell wall.” Molecular Microbiology 60(1) 93-107.
6. M. Inês Crisóstomo, Waldemar Vollmer, Arun Kharat, Silja Inhülen, Florian Gehre, Stephan Buckenmaier and Alexander Tomasz (2006). “Attenuation of penicillin resistance in a peptidoglycan O-acetyl transferase mutant of Streptococcus pneumoniae. Molecular Microbiology 61(6) 1497-1509.
7. Arun S. Kharat*, Marjolaine Noirclerc-Savoye, Evelyne Coursange, and Michel Blot (2006). Effect of limiting and excess glucose concentrations on transposition of IS1 in Escherichia coli K12.Acta Biochimica polonica 53(4) 729-738.
8. Marlene Damjanovich, Arun. S. Kharat, Waldemar Voller and Alexnader Tomasz (2007). The essential tacF gene is responsible for choline-dependent growth phenotype of Streptococcus pneumoniae. Journal of Bacteriology 189(19) 7105-7111. Both; Marlen Damjanovich and Arun S. Kharat should be treated as first author, contributed equally for this work, statement is available with publication.
9. Florian Gehre, Stephen Leib, Denis Grandgirard, Jurg Kummer, Angella Buhlmann, Franziska Simon, Rahel Gaumann, Arun S. Kharat, Martin Tauber, and Alexander Tomasz (2008). Essentail role of choline for pneumococcal virulence in an experimental model of Meningitis. In Press. Journal of Internal Medicine.
10. Arun S. Kharat, Florian Gehre, Waldemar Vollmer and Alexander Tomasz. Different pathways of choline metabolism – in two choline-independent strains of Streptococcus pneumoniae. Journal of Bacteriology, September 2008.
11. Arun S. Kharat, Helena Zemlickowa and Alexander Tomasz. Distribution of the srtA, srtB, srtC, and srtD sortase gene in Streptococcus pneumoniae: role of srtB srtC and srtD in mouse nasopharynx colonization. Manuscript in Preparation.
12. Arun S. Kharat and Alexander Tomasz. Functional and molecular characterization functional coupling of gryA-srtA in Streptococcus pneumoniae: a note on gyrA mediated polar effect on srtA activity. Manuscript in Preparation.

Publications of nucleotide submissions on www.ncbi.nlm.nih.gov/nucleotide

1. Arun S. Kharat and S. Mahadevan (2000). Shigella sonnei insertion sequence IS1, partial sequence. Accession number AF12368 available at http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=12044359
02. Arun S. Kharat and M. Blot (2000). Escherichia coli K12 ribosomal protein S12 (rpsL) gene, complete cds. Accession number AF312717. http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=11120596\
03. Arun S. Kharat and M. Blot (2000).Escherichia coli ribosomal protein S12 (rpsL) gene, rpsL150 allele, complete cds. Accession number AF312716. http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=1112059
04. Arun S. Kharat and S. Mahadevan (2000). Escherichia coli K12 antiterminator BglG (bglG) gene, partial cds. Accession number AF316396. http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=11096327
05. Arun S. Kharat and S. Mahadevan (1999). Shigella sonnei strain AK1 PhoU (phoU) and BglG (bglG) genes, partial cds. Accession number AF183894. http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=7159048.
06. Kharat,A.S. and Mahadevan,S (1999) Molecular characterization of the bglR and bglG genes of Shigella boydii (SBYR). Accession number 185096. http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=7385013.
07. Kharat,A.S. and Mahadevan,S (1999) Molecular characterization of the bglR and bglG genes of Shigella dysenteri (SFXR). Accession number 185095. http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=7385010.
08. Kharat,A.S. and Mahadevan,S (1999)Molecular characterization of the bglR and bglG genes of Shigella sonnei CR+. Accession number 185094. http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=7385007.
09. Kharat,A.S. and Mahadevan,S (1999) Molecular characterization of the bglR and bglG genes of Shigella sonnei CR-. Accession number AF185093. http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=7385004.
10. Kharat,A.S. and Mahadevan,S (1999). Cloning and characterization of the bglR, bglG region of Shigella sonnei SSOR. Accession number AF183895. http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=7159051.
Reviewed articles for, Journal of Experimental medicine, Molecular Microbiology, Infection and Immunity, Antimicrobial Agents and Chemotherapy and Microbial Drug Resistance.